output001.cpe.xml000075500000000000000000002437021147624423300076570 1.0 1291403624637 OMERO.editor PROTOCOL_ARCHIVE Sample This element describes the components with which the biological sample is prepared before the acquisition. 1287785118242 bhcho Slide Description of the protocol used to prepare the biological sample. This is a combination of Experiment, Protocol, and Label (cell type). STEP_GROUP Protocol Description of the protocol used to prepare the biological sample [[0]][[1]][[2]][[3]][[4]][[5]][[6]][[7]] Reference 0 OPTIONAL TEXT Fixation 1 OPTIONAL TEXT none Permeabilization 2 OPTIONAL TEXT none Substrate 3 OPTIONAL TEXT Glass Temperature 4 OPTIONAL NUMERIC ºC 20 Description 5 OPTIONAL TEXT Media was aspirated and replaced with Optimem containing Hoechst (at 0.5 microliter/mL concentration) followed by 45 minutes incubation before imaging. Author 6 OPTIONAL TEXT Title 7 OPTIONAL TEXT 3T3 RandTag Protocol 7 SINGLE_STEP Cell_Type Description of the cell type used to prepare the biological sample biological sample [[8]][[9]][[10]][[11]][[12]] Name 8 OPTIONAL TEXT 3T3-CDtag-CZ1E5 Organism 9 OPTIONAL TEXT Mus musculus atcc_number 10 OPTIONAL TEXT CRL-1658 cell_mesh_heading 11 OPTIONAL TEXT NIH 3T3 Cells cell_mesh_tree_number 12 OPTIONAL TEXT A11.251.210.700.550 SINGLE_STEP Labels STEP_GROUP Label The label describes the way the target is labelled with a fluorescent probe (ligand and dye) [[13]] ID 13 OPTIONAL TEXT 0 STEP_GROUP Ligand [[14]][[15]][[16]][[17]][[18]][[19]][[20]] source_organism_mesh_heading 14 OPTIONAL TEXT null source_organism_mesh_treenumber 15 OPTIONAL TEXT null supplier_part_number 16 OPTIONAL TEXT null substance_mesh_heading 17 OPTIONAL TEXT Green Fluorescent Proteins substance_mesh_treenumber 18 OPTIONAL TEXT D12.776.532.265 unigene_link 19 OPTIONAL TEXT null Name 20 OPTIONAL TEXT GFP SINGLE_STEP Target [[21]][[22]][[23]][[24]][[25]][[26]][[27]] source_organism_mesh_heading 21 OPTIONAL TEXT null source_organism_mesh_treenumber 22 OPTIONAL TEXT null supplier_part_number 23 OPTIONAL TEXT null substance_mesh_heading 24 OPTIONAL TEXT null substance_mesh_treenumber 25 OPTIONAL TEXT null unigene_link 26 OPTIONAL TEXT null Name 27 OPTIONAL TEXT unidentified SINGLE_STEP Dye The properties of the fluorescent dye [[28]][[29]][[30]][[31]][[32]][[33]] dye_mesh_heading 28 OPTIONAL TEXT Green Fluorescent Proteins excitation_max 29 OPTIONAL NUMERIC 488 emission_max 30 OPTIONAL NUMERIC 509 supplier 31 OPTIONAL TEXT null supplier_part_number 32 OPTIONAL TEXT null Name 33 OPTIONAL TEXT GFP SINGLE_STEP Label The label describes the way the target is labelled with a fluorescent probe (ligand and dye) [[34]] ID 34 OPTIONAL TEXT 1 STEP_GROUP Ligand [[35]][[36]][[37]][[38]][[39]][[40]][[41]] source_organism_mesh_heading 35 OPTIONAL TEXT null source_organism_mesh_treenumber 36 OPTIONAL TEXT null supplier_part_number 37 OPTIONAL TEXT null substance_mesh_heading 38 OPTIONAL TEXT null substance_mesh_treenumber 39 OPTIONAL TEXT null unigene_link 40 OPTIONAL TEXT null Name 41 OPTIONAL TEXT DsRed2-nuc SINGLE_STEP Target [[42]][[43]][[44]][[45]][[46]][[47]][[48]] source_organism_mesh_heading 42 OPTIONAL TEXT null source_organism_mesh_treenumber 43 OPTIONAL TEXT null supplier_part_number 44 OPTIONAL TEXT null substance_mesh_heading 45 OPTIONAL TEXT null substance_mesh_treenumber 46 OPTIONAL TEXT null unigene_link 47 OPTIONAL TEXT null Name 48 OPTIONAL TEXT nucleus SINGLE_STEP Dye The properties of the fluorescent dye [[49]][[50]][[51]][[52]][[53]][[54]] dye_mesh_heading 49 OPTIONAL TEXT Luminescent Proteins excitation_max 50 OPTIONAL NUMERIC 558 emission_max 51 OPTIONAL NUMERIC 583 supplier 52 OPTIONAL TEXT null supplier_part_number 53 OPTIONAL TEXT null Name 54 OPTIONAL TEXT DSRed2-nuc SINGLE_STEP Label The label describes the way the target is labelled with a fluorescent probe (ligand and dye) [[55]] ID 55 OPTIONAL TEXT 2 STEP_GROUP Ligand [[56]][[57]][[58]][[59]][[60]][[61]][[62]] source_organism_mesh_heading 56 OPTIONAL TEXT null source_organism_mesh_treenumber 57 OPTIONAL TEXT null supplier_part_number 58 OPTIONAL TEXT null substance_mesh_heading 59 OPTIONAL TEXT null substance_mesh_treenumber 60 OPTIONAL TEXT null unigene_link 61 OPTIONAL TEXT null Name 62 OPTIONAL TEXT Hoechst 33342 SINGLE_STEP Target [[63]][[64]][[65]][[66]][[67]][[68]][[69]] source_organism_mesh_heading 63 OPTIONAL TEXT null source_organism_mesh_treenumber 64 OPTIONAL TEXT null supplier_part_number 65 OPTIONAL TEXT null substance_mesh_heading 66 OPTIONAL TEXT null substance_mesh_treenumber 67 OPTIONAL TEXT null unigene_link 68 OPTIONAL TEXT null Name 69 OPTIONAL TEXT dna SINGLE_STEP Dye The properties of the fluorescent dye [[70]][[71]][[72]][[73]][[74]][[75]] dye_mesh_heading 70 OPTIONAL TEXT Hoechst 33342 excitation_max 71 OPTIONAL NUMERIC 354 emission_max 72 OPTIONAL NUMERIC 442 supplier 73 OPTIONAL TEXT null supplier_part_number 74 OPTIONAL TEXT null Name 75 OPTIONAL TEXT Hoechst 33342 SINGLE_STEP Label The label describes the way the target is labelled with a fluorescent probe (ligand and dye) [[76]] ID 76 OPTIONAL TEXT STEP_GROUP Ligand [[77]][[78]][[79]][[80]][[81]][[82]][[83]] source_organism_mesh_heading 77 OPTIONAL TEXT source_organism_mesh_treenumber 78 OPTIONAL TEXT supplier_part_number 79 OPTIONAL TEXT substance_mesh_heading 80 OPTIONAL TEXT substance_mesh_treenumber 81 OPTIONAL TEXT unigene_link 82 OPTIONAL TEXT Name 83 OPTIONAL TEXT SINGLE_STEP Target [[84]][[85]][[86]][[87]][[88]][[89]][[90]] source_organism_mesh_heading 84 OPTIONAL TEXT source_organism_mesh_treenumber 85 OPTIONAL TEXT supplier_part_number 86 OPTIONAL TEXT substance_mesh_heading 87 OPTIONAL TEXT substance_mesh_treenumber 88 OPTIONAL TEXT unigene_link 89 OPTIONAL TEXT Name 90 OPTIONAL TEXT SINGLE_STEP Dye The properties of the fluorescent dye [[91]][[92]][[93]][[94]][[95]][[96]] dye_mesh_heading 91 OPTIONAL TEXT excitation_max 92 OPTIONAL NUMERIC emission_max 93 OPTIONAL NUMERIC supplier 94 OPTIONAL TEXT supplier_part_number 95 OPTIONAL TEXT Name 96 OPTIONAL TEXT SINGLE_STEP Label The label describes the way the target is labelled with a fluorescent probe (ligand and dye) [[97]] ID 97 OPTIONAL TEXT STEP_GROUP Ligand [[98]][[99]][[100]][[101]][[102]][[103]][[104]] source_organism_mesh_heading 98 OPTIONAL TEXT source_organism_mesh_treenumber 99 OPTIONAL TEXT supplier_part_number 100 OPTIONAL TEXT substance_mesh_heading 101 OPTIONAL TEXT substance_mesh_treenumber 102 OPTIONAL TEXT unigene_link 103 OPTIONAL TEXT Name 104 OPTIONAL TEXT SINGLE_STEP Target [[105]][[106]][[107]][[108]][[109]][[110]][[111]] source_organism_mesh_heading 105 OPTIONAL TEXT source_organism_mesh_treenumber 106 OPTIONAL TEXT supplier_part_number 107 OPTIONAL TEXT substance_mesh_heading 108 OPTIONAL TEXT substance_mesh_treenumber 109 OPTIONAL TEXT unigene_link 110 OPTIONAL TEXT Name 111 OPTIONAL TEXT SINGLE_STEP Dye The properties of the fluorescent dye [[112]][[113]][[114]][[115]][[116]][[117]] dye_mesh_heading 112 OPTIONAL TEXT excitation_max 113 OPTIONAL NUMERIC emission_max 114 OPTIONAL NUMERIC supplier 115 OPTIONAL TEXT supplier_part_number 116 OPTIONAL TEXT Name 117 OPTIONAL TEXT SINGLE_STEP Label The label describes the way the target is labelled with a fluorescent probe (ligand and dye) [[118]] ID 118 OPTIONAL TEXT STEP_GROUP Ligand [[119]][[120]][[121]][[122]][[123]][[124]][[125]] source_organism_mesh_heading 119 OPTIONAL TEXT source_organism_mesh_treenumber 120 OPTIONAL TEXT supplier_part_number 121 OPTIONAL TEXT substance_mesh_heading 122 OPTIONAL TEXT substance_mesh_treenumber 123 OPTIONAL TEXT unigene_link 124 OPTIONAL TEXT Name 125 OPTIONAL TEXT SINGLE_STEP Target [[126]][[127]][[128]][[129]][[130]][[131]][[132]] source_organism_mesh_heading 126 OPTIONAL TEXT source_organism_mesh_treenumber 127 OPTIONAL TEXT supplier_part_number 128 OPTIONAL TEXT substance_mesh_heading 129 OPTIONAL TEXT substance_mesh_treenumber 130 OPTIONAL TEXT unigene_link 131 OPTIONAL TEXT Name 132 OPTIONAL TEXT SINGLE_STEP Dye The properties of the fluorescent dye [[133]][[134]][[135]][[136]][[137]][[138]] dye_mesh_heading 133 OPTIONAL TEXT excitation_max 134 OPTIONAL NUMERIC emission_max 135 OPTIONAL NUMERIC supplier 136 OPTIONAL TEXT supplier_part_number 137 OPTIONAL TEXT Name 138 OPTIONAL TEXT SINGLE_STEP Label The label describes the way the target is labelled with a fluorescent probe (ligand and dye) [[139]] ID 139 OPTIONAL TEXT STEP_GROUP Ligand [[140]][[141]][[142]][[143]][[144]][[145]][[146]] source_organism_mesh_heading 140 OPTIONAL TEXT source_organism_mesh_treenumber 141 OPTIONAL TEXT supplier_part_number 142 OPTIONAL TEXT substance_mesh_heading 143 OPTIONAL TEXT substance_mesh_treenumber 144 OPTIONAL TEXT unigene_link 145 OPTIONAL TEXT Name 146 OPTIONAL TEXT SINGLE_STEP Target [[147]][[148]][[149]][[150]][[151]][[152]][[153]] source_organism_mesh_heading 147 OPTIONAL TEXT source_organism_mesh_treenumber 148 OPTIONAL TEXT supplier_part_number 149 OPTIONAL TEXT substance_mesh_heading 150 OPTIONAL TEXT substance_mesh_treenumber 151 OPTIONAL TEXT unigene_link 152 OPTIONAL TEXT Name 153 OPTIONAL TEXT SINGLE_STEP Dye The properties of the fluorescent dye [[154]][[155]][[156]][[157]][[158]][[159]] dye_mesh_heading 154 OPTIONAL TEXT excitation_max 155 OPTIONAL NUMERIC emission_max 156 OPTIONAL NUMERIC supplier 157 OPTIONAL TEXT supplier_part_number 158 OPTIONAL TEXT Name 159 OPTIONAL TEXT SINGLE_STEP