The OME-TIFF Format
The OME-TIFF format was created to
maximize the respective strengths of OME-XML and TIFF. It takes advantage
of the rich metadata defined in OME-XML while retaining the pixels in
multi-page TIFF format for compatibility with many more applications.
An OME-TIFF dataset has the following characteristics:
- Image planes are stored within one multi-page TIFF file, or across
multiple TIFF files. Any image organization is feasible.
- A complete OME-XML metadata block describing the dataset is embedded
in each TIFF file’s header. Thus, even if some of the TIFF files in a
dataset are misplaced, the metadata remains intact.
- The OME-XML metadata block may contain anything allowed in a standard
- OME-TIFF uses the standard TIFF mechanism for storing one or more image
planes in each of the constituent files, instead of encoding pixels as
base64 chunks within the XML. Since TIFF is an image
format, it makes sense to only use OME-TIFF as opposed to OME-XML, when
there is at least one image plane.
OME-TIFF is supported by:
Public image repositories allowing image downloads as OME-TIFF
For detailed technical information on OME-TIFF, see the
There is further information about Extracting, Processing and Validating OME-XML available.
We also have some example code in Java for
extracting and modifying TIFF comments and converting other file formats
Lastly, some OME-TIFF Sample Data is available for
download, along with statistics comparing OME-TIFF and OME-XML with
various types of compression.