Hi Josh,
Sorry to cause confusion. The original image has dimensions (xy) 77092x156544. The ROI is around 24000x35000. The images are of adult mouse brain and they are generated using a slide scanner microscope. Users image at 20x so that they can count populations of cells. Sometimes processing of the image requires breaking up the original image into the individual tissue sections (the original file can be difficult to deal with). Currently users create these ROIs offline but I just wanted to see how feasible it was to do this on the server (it is rather slow but it actually handles it pretty well).
I was asking about pyramids because it seems like the display of the image generated from the ROI is quite slow. Maybe sticking to an offline solution then uploading would be better?
Cheers,
Dan.